ROTAROD ASSAY FOR MOTOR FUNCTION ACTIVITY OF WILD TYPE AND eIF2B MUTANT MICE
eIF2B mutant mouse is a Knock-In C57BL strain homozygous for R132H mutation in the eIF2B5 gene, encoding the catalytic subunit of eIF2B translation initiation factor. It serves as a mouse model for Vanishing White Matter (VWM) disease. The rotarod assay demonstrates impaired motor functions due to mutated eIF2B. For more information see Geva at al., Brain (2010).
LIVE IMAGING OF CELLS TRANSFECTED WITH FLUORESCENT tRNA SHOWING TRANSLATION INHIBITION BY PUROMYCIN
For additional details see Barhoom et al., NAR (2011).
FRET OF FLUORESCENT- LABELED tRNA IN LIVING CELLS
Live FRET in cells co-transfected with donor and acceptor fluorescent-labeled tRNAs. For additional details see Barhoom et al., NAR (2011).
LIVE IMAGING OF CELLS TRANSFECTED WITH FLUORESCENT- LABELED tRNA
Cells transfected with fluorescent tRNA. For additional details see Barhoom et al., NAR (2011).